Oral health can be a silent overseer of your overall well-being, and we're here to shed light on a connection that often goes unnoticed. Join me and Dr. Leslie Douglas, the Laboratory Director at DNA Connextions, as we unravel the potential health implications of root canals and other dental procedures. Dr. Douglas's expertise in molecular biology equips us with an in-depth understanding of PCR-based tests that identify and quantify a wide array of microorganisms in the oral cavity. This episode promises to open your eyes to the unseen world of oral pathogens and their possible link to systemic health issues.
My personal journey with dental health, including the aftermath of a root canal and mercury filling removal, is a testament to the profound impact dental procedures can have on one's vitality. Together with Dr. Douglas, we explore how PCR technology, often used for Lyme disease panels, can be a game-changer in assessing oral health. We address the controversial topic of dead tooth material potentially affecting the immune system, touching upon sobering concerns such as thyroid cancer. Through a comprehensive oral panel, we gain insights into the balance of microorganisms and draw parallels between oral and systemic health.
Dental materials are more than just tools of the trade; they can be harbingers of health issues if not chosen wisely. We discuss the importance of personalized testing to understand individual reactivity to metals and non-metals found in common dental products. By sharing real-life cases, like the decision to choose ceramic over titaniu
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00:00:02.403 --> 00:00:08.574 Hi everyone, it's me, sandy Cruz of Sandy K Nutrition, health and lifestyle queen.
00:00:08.574 --> 00:00:32.613 For years now, I've been bringing to you conversations about wellness from experts from all over the world, whether it be suggestions and how you can age better, suggestions in how you can age better, biohacking, alternative wellness these are conversations to help you live your best life.
00:00:32.613 --> 00:00:48.182 I want to live a long, healthy and vibrant life, never mind all those stigmas that, as we reach midlife and beyond, we're just going to shrivel up and die with some horrible disease.
00:00:48.182 --> 00:00:53.750 Always remember balanced living works.
00:00:53.750 --> 00:00:56.715 I really look forward to this season.
00:00:56.715 --> 00:01:04.671 Hi everyone, welcome to Sandy Kay Nutrition, health and Lifestyle Queen.
00:01:05.231 --> 00:01:08.885 Today with me I have Dr Leslie Douglas.
00:01:08.885 --> 00:01:25.852 She is the Laboratory Director for DNA Connections, and today we're going to be talking all about dental procedures and the potential for overgrowth of pathogenic bacteria.
00:01:25.852 --> 00:01:39.334 We are also going to be exploring my own results of a and I guess it's a 19 year old extraction of a root canal.
00:01:39.334 --> 00:01:47.873 Yeah, this is a big deal, you guys, and I'm going to pause for a second and say excuse my voice.
00:01:47.873 --> 00:01:53.209 I have never left my preamble to such last minute.
00:01:53.209 --> 00:02:10.122 This podcast releases on Monday and I'm recording my preamble on Sunday because I was away in Europe for 10 days, got home last Saturday, got COVID and have been really quite sick.
00:02:10.122 --> 00:02:18.145 So today I'm feeling pretty good, but my voice is just not the same yet, so please excuse my voice.
00:02:18.145 --> 00:02:25.248 It's also Thanksgiving today in Canada, so happy Thanksgiving to all of you who are in Canada and who celebrate.
00:02:25.248 --> 00:02:32.521 So I'm not going to spoil the conversation, but most of you know my story who listen to my podcast.
00:02:32.521 --> 00:02:55.295 You know that I had a root canal extracted without any major issues, but I had it extracted in the spring while I wanted to have it tested to see what was actually in there, and there's more and more evidence showing that these root canals can cause micro inflammation in the body.
00:02:55.295 --> 00:02:58.504 Since that root canal, I've had many issues.
00:02:58.504 --> 00:03:05.112 Obviously, I'm not going to get into this here, because I want you to listen to this conversation with Dr Leslie.
00:03:05.112 --> 00:03:21.366 I'm also going to ask for you to please share this, because it's just so important for people to be aware of risks for any procedure that we undergo any one.
00:03:21.366 --> 00:03:31.944 I had no idea of the risks of root canals and this is also why I will personally never get another root canal again.
00:03:31.944 --> 00:03:36.741 If I have to get rid of the tooth, I'll get rid of the tooth and that's that.
00:03:36.741 --> 00:03:39.586 So please share this.
00:03:39.586 --> 00:03:50.032 Also, I'm going to ask for you to rate and review my podcast wherever you listen, if it's on Spotify, if it's on Apple, apple especially.
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00:03:58.985 --> 00:04:04.032 This helps me to continue to get amazing guests each and every week.
00:04:04.860 --> 00:04:05.844 I am also a writer.
00:04:05.844 --> 00:04:08.882 I'll ask that you join my Substack.
00:04:08.882 --> 00:04:31.271 It is sandycruzesubstackcom S-A-N-D-Y-K-R-U-S-Esubstack, s-u-b-s-t-a-c-kcom, where you're going to actually be in a group where you're going to be able to read about explorative topics as it relates to wellness.
00:04:31.271 --> 00:04:57.723 I feel like we live in this world of telling, these days, where everyone's telling you this is healthy, this is not healthy, and so what I do is I explore many different topics of wellness relating to the body, the mind, the spirit, the soul, not just the science, because science is only one small little part of our wellness.
00:04:57.723 --> 00:04:59.605 So join me there.
00:04:59.605 --> 00:05:06.875 It will eventually be part of my new book that will be coming out at some point.
00:05:06.875 --> 00:05:09.108 It's sitting with a literary agent right now.
00:05:09.108 --> 00:05:14.300 Also, follow me on all of my social media channels.
00:05:14.300 --> 00:05:21.687 You're going to find me on any social media channel pretty much Sandy Kay Nutrition everywhere.
00:05:22.160 --> 00:05:28.485 And now let's cut on through to this amazing interview with Dr Leslie Douglas of DNA Connections.
00:05:28.485 --> 00:05:34.242 Hi, everyone, welcome to Sandy K Nutrition, health and Lifestyle Queen.
00:05:34.242 --> 00:05:37.829 Today with me I have Dr Leslie Douglas.
00:05:37.829 --> 00:05:41.802 She is a geneticist Did I say that right?
00:05:41.802 --> 00:05:42.944 Geneticist?
00:05:42.944 --> 00:05:53.752 It's so hard to say that and molecular biologist, and she is the principal investigator and laboratory director for DNA Connections.
00:05:54.439 --> 00:06:07.696 Dna Connections is a top US-based company focusing on bacterial, viral, fungal and parasitic molecular-based detection assays.
00:06:07.696 --> 00:06:30.803 Dr Douglas' professional focus is the research and development of PCR-based testing, direct DNA, including Lyme disease tests, apoe tests, genotyping tests, comprehensive oral tests, gluten sensitivity tests and propensity tests.
00:06:30.803 --> 00:06:43.089 But today we are going to be talking mostly about dental procedures and the potential for overgrowth of pathogenic bacteria.
00:06:43.089 --> 00:07:13.132 Now, most of you have been following me for a while know that I recorded a while back with Dr Kelly Blodgett about biological dentistry and I'm so fortunate that through him I found DNA connections and I really feel honored to be able to be just introduced to people who are doing such amazing things and changing health and wellness as we know it.
00:07:13.132 --> 00:07:26.403 And today we're going to talk a little bit about some of my own test results, but we're going to get into that later with Dr Leslie Douglas and with that, welcome, Leslie.
00:07:26.403 --> 00:07:27.446 Can I call you Leslie?
00:07:27.466 --> 00:07:29.610 Absolutely.
00:07:29.610 --> 00:07:31.870 Thank you so much, thanks for having me.
00:07:32.201 --> 00:07:33.848 I'm happy to have you on.
00:07:33.848 --> 00:07:48.781 I mean, you are so knowledgeable about all these tests, so we want to know more, and people who have been following me just want to know more and understand more.
00:07:48.781 --> 00:07:52.230 So who better to discuss this with than you?
00:07:52.230 --> 00:08:10.500 So let's get into your background first, because I always like to hear about how you got into this yeah.
00:08:10.519 --> 00:08:20.086 So during undergraduate I was really into the sciences I ended up with I was raised in hawaii and I ended up with a bachelor's in biology from the university of hawaii knew I wanted to go into some sort of molecular based research.
00:08:20.086 --> 00:08:23.449 I'm just know that type of very analytical person.
00:08:23.449 --> 00:08:27.755 So I then went on to graduate school.
00:08:27.755 --> 00:08:36.650 I received a PhD in genetics and molecular biology and took it from there.
00:08:36.650 --> 00:08:53.067 I was at DNA Connections just a little over 10 years ago and right now we have plenty of panels, but at that time it was just some smaller panels and we started working on a line panel.
00:08:53.067 --> 00:09:04.746 So the panel that we're going to be talking about today is the comprehensive oral and, as we were discussing, you know, people don't have the idea that dentistry can be bad for you.
00:09:04.746 --> 00:09:25.604 Some dentistry can affect people very adversely, in addition to attracting and being just a site of persistence of my firms that are able to create biofilms and congregate on these restorations and inside the oral cavity, on these restorations and inside the oral cavity.
00:09:25.624 --> 00:09:32.408 Some of these organisms are commonly found in the mouth, are generally benign.
00:09:32.408 --> 00:09:49.332 Some of these organisms have been scientifically linked to chronic conditions and different characteristics of the organisms benign to entirely pathogenic.
00:09:49.332 --> 00:09:55.984 You know we find them all in the oral cavity.
00:09:55.984 --> 00:09:59.116 So the panel that we're going to be talking about today is the comprehensive oral.
00:09:59.116 --> 00:10:00.484 It's a PCR-based panel.
00:10:00.484 --> 00:10:07.955 It has 88 microbes, 88 potential pathogens, as 88 microbes, 88 external pathogens, inclusive of bacteria, viruses, parasites and fungi.
00:10:07.955 --> 00:10:13.043 Each one is what we call a direct testing method.
00:10:13.043 --> 00:10:25.110 So we're looking for the DNA of the organism itself, we target live cellular material and we can basically test any sample from the oral cavity.
00:10:25.110 --> 00:10:28.559 And we can basically test any sample from the oral cavity.
00:10:28.559 --> 00:10:42.009 We can do teeth blood, permutational blood, which we can get into in a minute, sex-specific, or we even have a full mouth serving with a product called Super Floss whole panel.
00:10:42.028 --> 00:10:59.339 You just floss with the specialized product that's in our comprehensive kit send it back to us and we can do a full mouth survey of pathogens that we find in your mouth.
00:10:59.339 --> 00:11:09.822 I think it's a good place to start to.
00:11:09.822 --> 00:11:20.802 You know, just say, for those of you who are not in the wellness industry, understanding that we have bacteria everywhere is really important, and it's not necessarily that oh no, she has bacteria, bacteria is everywhere, it's everywhere.
00:11:20.802 --> 00:11:31.755 When you say pathogenic, I know you're speaking of the overgrowth of specific bacterias that, in an overgrowth stage, can be harmful.
00:11:31.755 --> 00:11:33.297 Right, does that make sense?
00:11:34.379 --> 00:11:43.451 Yeah, well, I mean every organism in each of the organisms that were specially selected for this comprehensive oral panel.
00:11:43.451 --> 00:11:47.421 It's based on the characteristics of the organism.
00:11:47.421 --> 00:11:58.135 So we go from denying commonly found in the oral cavity to is it microbial resistant?
00:11:58.135 --> 00:12:01.403 Is it multi-microbial resistant?
00:12:01.403 --> 00:12:06.892 Does it produce toxins, you know, and it killed you?
00:12:06.892 --> 00:12:14.789 Uh, so it goes from benign to entirely pathogenic, that it doesn't belong in the oral cavity whatsoever.
00:12:14.789 --> 00:12:46.030 Okay, so when you're dealing with these particular particular restorations that have been done that in the industry are being corrected or extracted in your case, there's a certain ways that are recommended to treat the area to keep these organisms from becoming a systemic issue, for those that it's already a systemic issue.
00:12:46.030 --> 00:12:49.815 Then there's organisms that are involved in chronic conditions.
00:12:49.815 --> 00:13:06.136 You know you've got your campers or yourcus species that are related to infection, chronic infection.
00:13:06.136 --> 00:13:11.207 Certain streptococcus species are related to cardiac issues.
00:13:11.207 --> 00:13:22.687 So for you know these chronic conditions where people just they just don't understand why they are suffering from it.
00:13:22.687 --> 00:13:26.581 It could be just borax in your mouth, mm-hmm.
00:13:28.405 --> 00:13:32.193 I think it's before we get into all of this.
00:13:32.193 --> 00:13:33.056 It's a good.
00:13:33.056 --> 00:13:40.870 For those of you who maybe don't know what happened, I'll try and make it kind of quick.
00:13:40.870 --> 00:13:44.596 But I had a root canal extracted.
00:13:44.596 --> 00:13:51.966 It kind of quick, but I had a root canal extracted and what prompted me was I had this root canal for almost 19 years.
00:13:51.986 --> 00:14:01.725 So to backtrack, after the birth of my second child, one day my three bottom teeth, in the very front, they just crumbled.
00:14:01.725 --> 00:14:05.693 This is why nutrients are really important when you're pregnant.
00:14:05.693 --> 00:14:12.311 This is long before I was a practitioner and then I had a cracked tooth right around the same time.
00:14:12.311 --> 00:14:16.831 So obviously I had a weakened system after giving birth.
00:14:16.831 --> 00:14:19.508 There's no other way to describe it.
00:14:19.508 --> 00:14:23.871 Your teeth don't just crumble in your mouth unless you have nutrient deficiencies period.
00:14:23.871 --> 00:14:25.403 You can't explain that.
00:14:25.403 --> 00:14:30.514 So I was told you have to have a root canal, right?
00:14:30.514 --> 00:14:33.461 This is going back almost 19 years.
00:14:33.461 --> 00:14:37.791 So I did it and then fast forward.
00:14:38.072 --> 00:14:46.753 Since then I've been diagnosed with mitral valve prolapse, with mild regurgitation, which I don't have in my family.
00:14:46.753 --> 00:14:49.927 There's no genetics, but a heart murmur.
00:14:49.927 --> 00:14:52.272 This was after I had the root canal.
00:14:52.272 --> 00:14:54.101 I've also had thyroid cancer.
00:14:54.101 --> 00:14:57.708 I've also had a breast fibroadenoma.
00:14:57.708 --> 00:15:00.274 So I've had a number of things.
00:15:01.922 --> 00:15:05.657 I am a big believer that it's always going to be multifactorial.
00:15:05.657 --> 00:15:23.350 There were a lot of things going on in my life, so you can't just say it's one thing, but did this root canal contribute to these weaknesses that manifested into issues, right?
00:15:23.350 --> 00:15:32.548 So then I went and had a thermography full body thermography which showed inflammation in the area where I had this root canal.
00:15:32.548 --> 00:15:34.833 That was this year, in 2024.
00:15:34.833 --> 00:15:42.326 So I thought, okay, it's time now I feel like I'm in a healthy state.
00:15:42.326 --> 00:15:50.236 I think it's time to take this out, even though I had no direct, obvious symptoms.
00:15:51.981 --> 00:15:53.067 So I extracted it.
00:15:53.067 --> 00:16:01.567 And here we are today where we're going to talk about what we found, which I think so many people are interested in.
00:16:01.567 --> 00:16:07.851 And the one big question, leslie, is how do you even test?
00:16:07.851 --> 00:16:31.350 So what we did, my dentist made sure that it was put in a, so I had two roots plus the tooth in a sterile bag that I put in the freezer and then I waited for the kit and then I sent it off in the kit, like, how do you get DNA from like an old, like it was sitting in the freezer?
00:16:31.350 --> 00:16:35.750 So this is all very fascinating to me and I think we should explain it.
00:16:37.181 --> 00:16:40.366 Well, it's about you know.
00:16:40.366 --> 00:16:42.010 I mean there's some people you know.
00:16:42.010 --> 00:16:44.927 I mean maybe when you went to your dentist and said, you know, I'd like to take this tooth that has been treated.
00:16:44.927 --> 00:16:45.710 I mean there's some people you know.
00:16:45.710 --> 00:16:48.200 I mean maybe when you went to your dentist and said, you know, I'd like to take this tooth that has been treated.
00:16:48.200 --> 00:16:57.716 I mean it has a root canal, it's dead but it's stable, and your dentist must have looked at you like what you want to pull out a perfectly good tooth.
00:16:57.716 --> 00:17:07.394 I mean there are millions of practitioners that are called endodontists and they make a career in root canal services.
00:17:09.782 --> 00:17:11.628 So what is a root canal?
00:17:11.628 --> 00:17:32.910 Okay, a root canal is when either the tooth is okay let me say, I'm a PhD, I'm not a dentist okay, so from my understanding, a root canal is when the tooth is decayed to the point that to save it they need to kill it, and when they save it, they're essentially saving the structure.
00:17:32.910 --> 00:17:44.375 So what they do is they access the tooth and they use kind of like these little pipe cleaners and they put them down into the canals of the teeth and they sort of twizzle it.
00:17:44.375 --> 00:17:48.428 And they pull out the canals of the teeth and they sort of twizzle it right and they pull out the blood supply and the nerve.
00:17:48.428 --> 00:17:52.534 So it is basically it's it's difficult, okay.
00:17:52.534 --> 00:18:09.662 And then they with different types of material, kind of in a generalized category called better purchase, okay, uh oh, before they fill it with better purchase, they actually use dilute bleach to clean out those canals.
00:18:09.662 --> 00:18:18.067 They literally take bleach off the shelf, they dilute it and they clean out the canals and then they stuff them full of this product.
00:18:18.067 --> 00:18:21.742 It's like a rubber or some sort of resin.
00:18:21.742 --> 00:18:25.893 There's different variations of them called gutter perchance.
00:18:25.893 --> 00:18:34.268 So it's a really essentially long filling along the length of that tooth in the roots and then you put a crown on top.
00:18:34.268 --> 00:18:43.909 Sometimes they need to do a buildup where they put in a metal post for more structure, because now it's dead bone and they put a crown on top.
00:18:44.911 --> 00:18:51.042 So some people are absolutely fine in theory with a root canal procedure.
00:18:51.042 --> 00:19:00.642 They're healthy, it doesn't bother them, it doesn't, you know, cause any kind of inflammatory issues.
00:19:00.642 --> 00:19:03.758 Okay, that would be, I guess, a lucky percent.
00:19:03.758 --> 00:19:05.471 But think about it.
00:19:05.471 --> 00:19:15.205 So this dead corn material is now basically disrupting your immune system.
00:19:15.205 --> 00:19:18.460 Your immune system is kind of like wow, sandy, what is that?
00:19:18.460 --> 00:19:21.820 What is that in your lower right causing your inflammation.
00:19:21.820 --> 00:19:27.501 It's always kind of on the immune system's radar to take care of it.
00:19:27.501 --> 00:19:36.182 Now, because your immune system was entertained by that, is that when you develop thyroid cancer, right.
00:19:36.182 --> 00:19:40.029 So you know it's self versus non-self.
00:19:40.029 --> 00:19:44.582 That is a big pile of non-self materials, dead as dead.
00:19:44.582 --> 00:19:45.904 It should be removed.
00:19:46.025 --> 00:19:55.779 But, like I'll tell you, I had a crowd at my dentist not that long ago and it was giving me sensitivity, and immediately he's like, oh, maybe you need a root canal.
00:19:55.779 --> 00:19:59.096 I said no, no, if I need a root canal, you're pulling it.
00:19:59.096 --> 00:20:00.236 I would never get a root canal.
00:20:00.236 --> 00:20:02.618 That's my personal from what I know.
00:20:02.618 --> 00:20:05.170 That's my personal belief.
00:20:05.170 --> 00:20:07.454 I would never do that, anyway.
00:20:07.454 --> 00:20:25.411 So there are many, many people like you that talk to me, that talk to Dr Bludgett that is becoming more schooled in holistic dentistry and the restorations in your mouth can affect your entire immune system, your immune balance and your overall health.
00:20:27.013 --> 00:20:28.277 So what we do?
00:20:28.277 --> 00:20:39.582 So we have this comprehensive oral panel and it's like I said, it's a PCR panel, so we target the DNA of the organisms itself.
00:20:39.582 --> 00:20:53.336 There's 88 organisms, like I said, and some dentists keep our kids in their offices and some people like you, they care about us and they order a kit.
00:20:53.336 --> 00:20:57.278 You can have your kit on hand when you go to the dentist's office.
00:20:57.278 --> 00:21:05.339 It comes with everything that they need for collection, whether it's going to be bone, tooth, blood tissue.
00:21:05.339 --> 00:21:19.352 It comes with sterile plastic where the sample is deposited into the sterile plastic where it is packaged up, depending on the sample type, according to the instructions that come in the kit.
00:21:19.352 --> 00:21:23.601 It includes prepaid shipping to send it back to us by UPMs.
00:21:24.481 --> 00:21:29.256 If you can't ship back the sample immediately, then you freeze it.
00:21:29.256 --> 00:21:35.986 The freezing process basically just stalls any bacterial overgrowth.
00:21:35.986 --> 00:21:44.957 So I have people that hear about this procedure and they say, oh, my dentist put it in a Ziploc bag and it's sitting on my counter in the kitchen.
00:21:44.957 --> 00:21:46.449 Like, yeah, that's not going to work.
00:21:46.449 --> 00:21:53.914 Ziplocs aren't sterile and with room temperature you'll have bacterial overgrowth.
00:21:53.914 --> 00:21:55.800 So what's there will just flourish.
00:21:55.800 --> 00:22:00.296 So we recommend freezing it until you can send it back to our laboratory.
00:22:00.296 --> 00:22:37.227 Once it comes back to the laboratory, it's checked into the lab and we go through several stages of extraction as well as quantification of the resultant, this PCR-based plate to amplify individually the organisms that are on the panel.
00:22:37.227 --> 00:22:42.015 The panel results which we're going to go over.
00:22:42.015 --> 00:23:06.657 Your specific results show not only how much of the organism, which organisms we detected, how much of the organism we detected, and the algorithm takes into account, like we said, the characteristics of the organism and its overall potential impact in your oral cavity and systemically as well.
00:23:06.657 --> 00:23:08.541 It's a lot.
00:23:08.869 --> 00:23:09.571 It is a lot.
00:23:09.571 --> 00:23:10.474 It is a lot, it is a lot.
00:23:10.474 --> 00:23:17.079 Now I have to ask is it possible for it to be wrong like false positives?
00:23:17.079 --> 00:23:18.663 I know you explained.
00:23:18.663 --> 00:23:23.094 Obviously, if the sample is tainted, that's a factor.
00:23:23.094 --> 00:23:37.919 But if the sample was immediately put into a sterile bag and then put into the freezer, if you follow the instructions, is it possible to get wrong results?
00:23:37.939 --> 00:23:41.935 Sure, so that's a great question.
00:23:41.935 --> 00:24:02.944 So PCR, which is the polymerase chain reaction, which is what we do to detect the DNA of the individual organisms, it's inherently specific, and so what that means is that if the organism is present, the reaction proceeds and you're getting positive.
00:24:02.944 --> 00:24:05.438 And then it's again how positive were you?
00:24:05.438 --> 00:24:09.945 If the organism is not present, there is no reaction that proceeds.
00:24:09.945 --> 00:24:18.624 And you know there's a lot of things that we do to ensure that we don't get false positives.
00:24:18.624 --> 00:24:27.180 Pcr has a starting point, and that's kind of like the secret sauce, as our owner says.
00:24:27.180 --> 00:24:34.001 The starting point are called primers and the primers sit down on the DNA of the organism itself.
00:24:34.001 --> 00:24:36.257 That's where the reaction begins.
00:24:36.257 --> 00:24:43.887 So when that DNA is not present, the primers don't anneal, is what they call it, so the reaction does not proceed.
00:24:44.650 --> 00:24:45.994 Okay, that makes sense.
00:24:45.994 --> 00:24:46.898 That makes sense.
00:24:46.898 --> 00:24:50.471 I'm not a molecular biologist but I think I get what you're saying.
00:24:50.471 --> 00:24:53.933 I think I get it.
00:24:53.933 --> 00:25:17.757 Now here's the other thing, and I know people are going to ask okay, this is kind of getting confusing to ask this question, but how do we know if these pathogens are dormant or active?
00:25:17.757 --> 00:25:20.862 Would it be the amount that is found?
00:25:20.862 --> 00:25:28.413 Or like I don't know if I'm asking the question correctly Like, is it possible for these pathogens so they're in?
00:25:28.413 --> 00:25:30.875 Like, is it possible for these pathogens so they're in, basically in levels?
00:25:30.875 --> 00:25:40.243 Because you guys and we will show this, so if you want to watch it on YouTube or on Rumble, I will have a link to all of this.
00:25:40.243 --> 00:25:45.047 But you provide in the report levels.
00:25:45.047 --> 00:25:53.906 Does that mean how active that is In that DNA, in that piece of DNA, that tooth?
00:25:55.030 --> 00:25:58.618 It's more quantity, it's more quantity.
00:25:58.618 --> 00:26:01.826 So we have another panel.
00:26:01.826 --> 00:26:03.288 This might help.
00:26:03.288 --> 00:26:04.291 We have another panel.
00:26:04.291 --> 00:26:05.694 It's the Lyme disease panel.
00:26:05.694 --> 00:26:18.519 Okay, so Lyme disease is called by bacteria and when we detect a positive or say a negative, when we detect a positive, people ask well, is it active infection?
00:26:18.519 --> 00:26:25.423 So we target the DNA of Lyme organisms.
00:26:25.423 --> 00:26:38.731 Organisms, so the so pcr, okay, the primers.
00:26:38.731 --> 00:26:42.135 The start point of the reaction it's looking for a target, a target within the entire genome of this organism.
00:26:42.135 --> 00:26:53.634 So the genome of the organism that causes Lyme, borrelia burgdorferi, is a million base pairs and that's small, genome-wise that's small.
00:26:53.634 --> 00:27:06.935 But within that million base pairs we target between 250, about 250,000, 250 and about 1,200 base pairs.
00:27:06.935 --> 00:27:31.604 So the idea of it being like degraded, sloughed off DNA from a dead and kind of, you know, decaying organism, it's just statistically unlikely that the 250 base pairs out of a million that we're targeting is still intact and amplifiable.
00:27:31.604 --> 00:27:33.336 It's just statistically unlikely.
00:27:33.617 --> 00:27:39.855 Okay, that's one of the reasons why PCR is so specific it's presence or absence.
00:27:39.855 --> 00:27:44.029 If the organism isn't there, then it's not going to proceed.
00:27:44.029 --> 00:27:47.596 So if the organism wasn't in the sample provided, it's not going to proceed.
00:27:47.596 --> 00:27:49.259 Okay, okay, it's not going to proceed.
00:27:51.243 --> 00:27:57.123 Now we know that this was extracted from my mouth.
00:27:57.123 --> 00:28:06.539 Does this mean that the organisms are gone, the microbes are gone, when it's extracted?
00:28:06.539 --> 00:28:09.961 Or does this mean it's gone systemic into my body and some of them are gone when it's extracted?
00:28:09.961 --> 00:28:12.371 Or does this mean it's gone systemic into my body and some of them are gone?
00:28:12.371 --> 00:28:15.616 Like what does it mean when we remove it?
00:28:15.616 --> 00:28:17.140 How do we know?
00:28:18.402 --> 00:28:30.119 So the first thing that you're doing when you remove a root canal tube is that you're removing that non-cell, that foreign object that's been entertaining your immune system.
00:28:30.119 --> 00:28:31.163 So that's number one.
00:28:31.163 --> 00:28:37.577 So immediately your immune system is like it's like a relief, it's gone, yeah.
00:28:37.577 --> 00:28:41.316 So we do recommend that.
00:28:41.316 --> 00:28:51.586 You know any dentist can do extractions, any dentist can, you know, can perform cavitational surgery.
00:28:51.586 --> 00:29:18.628 But we do recommend there are groups and what they call holistic dental practitioners that understand, like Dr Bledgett, who understand that when you remove a tooth or when you clean out a cavitation, that there is a possibility that the organisms can escape systemically.
00:29:18.628 --> 00:29:23.782 So they clean it out with lasers, they clean it out with ozone therapy.
00:29:24.611 --> 00:29:28.922 It's not just kind of pulling the tooth and you're good.
00:29:28.922 --> 00:29:30.692 It's not just kind of pulling the tooth and you're good.
00:29:30.692 --> 00:29:40.296 It's about the healing process as well as the procedure to remove or replace or correct for your dentistry.
00:29:40.296 --> 00:29:46.307 When you have metal fillings replaced, you certainly don't want.
00:29:46.307 --> 00:29:55.915 When they're grinding out that metal filling that contains mercury, you don't want to be and the dentist doesn't want giving any medical advice in this podcast.
00:30:12.390 --> 00:30:20.105 We are simply just providing you with information so that you can go and explore and educate yourself in this area.
00:30:20.105 --> 00:30:22.317 That's the whole reason I do these podcasts.
00:30:22.317 --> 00:30:35.316 What I did in advance was kind of made sure that I was cleaned up, let's just say, before I had the extraction.
00:30:35.316 --> 00:30:55.678 So I'm a big believer as a practitioner in toxic load and toxic burden and I do believe and this is my opinion that when you do have a procedure like this, you want to be in a healthy state.
00:30:55.678 --> 00:30:59.029 You don't want that bucket to overflow.
00:30:59.029 --> 00:31:07.285 So I have my own protocol on what I did before I had the extraction.
00:31:07.285 --> 00:31:10.799 Now I have a dental surgeon here where I am.
00:31:10.799 --> 00:31:18.509 He's not a biological dentist per se, but I trust him and I think trust is very important.
00:31:18.509 --> 00:31:26.621 And biological dentistry I think more and more are coming to be, but there's not a ton of them, right?
00:31:26.621 --> 00:31:29.118 I would love to see more.
00:31:29.118 --> 00:31:38.473 So taking accountability for your own wellness before you undergo a procedure like that, I think is really important, don't you?
00:31:39.877 --> 00:31:43.356 Absolutely, absolutely, and your analogy is great.
00:31:43.356 --> 00:31:54.199 Like you don't want that to overflow, you said that you, you know, had this root canal, lived with this root canal, but then you know, like you said, prepared yourself.
00:31:54.199 --> 00:31:55.182 That's really wise.
00:31:55.910 --> 00:31:58.817 Yeah, actually, leslie, I did a lot Like.
00:31:58.817 --> 00:32:21.238 I was one of those like people who, okay, this is so weird and I have to just mention this in that whole body thermography not only did they see this, they saw, you know, some inflammation, thermogenic patterns in the lymph, which kind of makes sense right With a root canal, and this is really weird.
